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p0704s critical  (New England Biolabs)


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    New England Biolabs p0704s critical
    P0704s Critical, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 7078 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p0704s+critical/PNGase+F/pm37683630-521-154-150
    Average 99 stars, based on 7078 article reviews
    p0704s critical - by Bioz Stars, 2026-09
    99/100 stars

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    Recombinant:

    Article Title: Usp25-Erlin1/2 activity limits cholesterol flux to restrict virus infection
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Usp25 Abcam Cat# ab187156 Anti-SMURF1 Santa Cruz Cat# sc-100616 Anti-Usp28 Abcam Cat# ab126604; RRID: AB_11127442 Anti-GAPDH Abcam Cat# ab8245; RRID: AB_2107448 Anti-PB2 Thermo Fisher Scientific Cat# PA5-32220; RRID: AB_2549693 Anti-HA Abcam Cat# ab119966; RRID: AB_10899358 Anti-NS1 Santa Cruz Cat# sc-130568; RRID: AB_2011757 Anti-NP Abcam Cat# ab128193; RRID: AB_11143769 Anti-RIG-I Abcam Cat# ab180675 Anti-MAVS Cell Signaling Technology Cat# 3993; RRID: AB_823565 Anti-TBK1 Cell Signaling Technology Cat# 3504; RRID: AB_2255663 Anti-Phospho-TBK1 (Ser172) Cell Signaling Technology Cat# 5483; RRID: AB_10693472 TRAF3 Abcam Cat# 36988 Anti-IRF3 Cell Signaling Technology Cat# 4302; RRID: AB_1904036 Anti-Phospho-IRF3 (Ser386) Cell Signaling Technology Cat# 37829; RRID: AB_2799121 Anti-IRF7 Cell Signaling Technology Cat# 4920; RRID: AB_2127551 Anti-STAT1 Cell Signaling Technology Cat# 9172; RRID: AB_2198300 Anti-Phospho-STAT1 (Tyr701) Cell Signaling Technology Cat# 9167; RRID: AB_561284 Anti-STAT2 Cell Signaling Technology Cat# 4594; RRID: AB_2271323 IFIT1 Abcam Cat# 236256 Anti-Phospho-STAT2 (Tyr690) Cell Signaling Technology Cat# 88410; RRID: AB_2800123 Anti-Beclin 1 Cell Signaling Technology Cat# 3495; RRID: AB_1903911 Anti-ATG 5 Cell Signaling Technology Cat 12994; RRID: AB_2630393 Anti-ATG 12 Cell Signaling Technology Cat# 4180; RRID: AB_1903898 Anti-LC 3 Cell Signaling Technology Cat# 2775; RRID: AB_915950 Anti-LAMP1 Cell Signaling Technology Cat# 9091; RRID: AB_2687579 Anti-LAMP2 Abcam Cat# 25631 Anti-Capthesin B Cell Signaling Technology Cat#31718; RRID: AB_2687580 Anti-Capthesin D Abcam Cat# ab198326 Anti-Rab 11 Cell Signaling Technology Cat# 5589; RRID: AB_10693925 Anti-Rab 8b Thermo Fisher Scientific CAT# PA5-67354; RRID: AB_2664303 Anti-Flag-HRP Sigma Aldrich Cat# A8592; RRID: AB_439702 Anti-HA-HRP Abcam Cat# ab128131; RRID: AB_11143947 Anti-c-Myc Roche Cat# 11667203001; RRID: AB_390911 Bacterial and Virus Strains A/California/07/2009(H1N1 pandemic) Jahan et al.9 N/A A/Oklahoma/309/06 (H3N2) Fan et al.43 N/A A/Hong Kong/1073/99 (H9N2) Chan et al.44 N/A A/Vietnam/1203/2004 (H5N1) Herfst et al.45 N/A A/Shanghai/02/2013 (H7N9) Mok et al.46 N/A Human coronavirus 229E ATCC VR-740 BetaCoV/Hong Kong/VM20001061/2020 Lv et al.47 N/A ZIKV (NC-14-5132) Lan et al.16 and Li et al.17 N/A Chemocompetent E. coli DH5a Thermo Fisher Scientific Cat# 18265017 (Continued on next page) e1 Developmental Cell 58, 2495–2509.e1–e6, November 20, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins DMEM Thermo Fisher Scientific Cat# 10569010 HEPES Thermo Fisher Scientific Cat# 15630130 Trypsin-EDTA Thermo Fisher Scientific Cat# 25200072 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122 Fetal Bovine Serum (FBS) Thermo Fisher Scientific Cat# 10500064 Phosphate-buffered saline (PBS) Thermo Fisher Scientific Cat# 14040133 Puromycin InvivoGen Cat# ant-pr-1 Hygromycin B Thermo Fisher Scientific Cat# 10687010 Triton X-100 Sigma Aldrich Cat# 93443 Complete protease inhibitor cocktail Roche Cat# 11836145001 Normal Goat Serum (NGS) Thermo Fisher Scientific Cat# 31873 MG132 Sigma Aldrich Cat# M7449 TransITLT1 transfection reagent Mirus Cat# MIR2304 Lipofectamine 3000 Thermo Fisher Scientific Cat# L3000008 Polyethylenimine (PEI) Polysciences Cat# 23966-1 DharmaFECT1 Dharmacon Cat# T-2001-03 Octadecyl Rhodamine B Chloride (R18) Thermo Fisher Scientific Cat# O246 Fix Perm Kits BD PharMingen Cat# 554714 TPCK-Trypisn Thermo Fisher Scientific Cat# 20233 T4 ligase New England Biolabs Cat# M0202S Endo H New England Biolabs Cat# P0702S PNGase F New England Biolabs Cat# P0704S Critical Commercial Assays TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.USP28.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.SMURF1.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.BECN1.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.MAP1LC3B.13 PrimeScript RT Reagent Kit TAKARA Cat# RR037A MiniBEST Universal RNA Extraction Kit TAKARA Cat# 9767 MiniBEST Viral RNA Extraction Kit TAKARA Cat# 9766 TB Green Premix Ex Taq II (Tli RNase H Plus) TAKARA Cat# RR820A LEGENDplex Human Anti-Virus Response Panel Biolegend Cat# 740390 LEGENDplex Human Proinflammatory Chemokine Panel 1 Biolegend Cat# 740984 Deposited Data Proteomics; PRIDE database This study Accession: PXD034797 Experimental Models: Cell Lines ..

    Article Title: Usp25-Erlin1/2 activity limits cholesterol flux to restrict virus infection.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Usp25 Abcam Cat# ab187156 Anti-SMURF1 Santa Cruz Cat# sc-100616 Anti-Usp28 Abcam Cat# ab126604; RRID: AB_11127442 Anti-GAPDH Abcam Cat# ab8245; RRID: AB_2107448 Anti-PB2 Thermo Fisher Scientific Cat# PA5-32220; RRID: AB_2549693 Anti-HA Abcam Cat# ab119966; RRID: AB_10899358 Anti-NS1 Santa Cruz Cat# sc-130568; RRID: AB_2011757 Anti-NP Abcam Cat# ab128193; RRID: AB_11143769 Anti-RIG-I Abcam Cat# ab180675 Anti-MAVS Cell Signaling Technology Cat# 3993; RRID: AB_823565 Anti-TBK1 Cell Signaling Technology Cat# 3504; RRID: AB_2255663 Anti-Phospho-TBK1 (Ser172) Cell Signaling Technology Cat# 5483; RRID: AB_10693472 TRAF3 Abcam Cat# 36988 Anti-IRF3 Cell Signaling Technology Cat# 4302; RRID: AB_1904036 Anti-Phospho-IRF3 (Ser386) Cell Signaling Technology Cat# 37829; RRID: AB_2799121 Anti-IRF7 Cell Signaling Technology Cat# 4920; RRID: AB_2127551 Anti-STAT1 Cell Signaling Technology Cat# 9172; RRID: AB_2198300 Anti-Phospho-STAT1 (Tyr701) Cell Signaling Technology Cat# 9167; RRID: AB_561284 Anti-STAT2 Cell Signaling Technology Cat# 4594; RRID: AB_2271323 IFIT1 Abcam Cat# 236256 Anti-Phospho-STAT2 (Tyr690) Cell Signaling Technology Cat# 88410; RRID: AB_2800123 Anti-Beclin 1 Cell Signaling Technology Cat# 3495; RRID: AB_1903911 Anti-ATG 5 Cell Signaling Technology Cat 12994; RRID: AB_2630393 Anti-ATG 12 Cell Signaling Technology Cat# 4180; RRID: AB_1903898 Anti-LC 3 Cell Signaling Technology Cat# 2775; RRID: AB_915950 Anti-LAMP1 Cell Signaling Technology Cat# 9091; RRID: AB_2687579 Anti-LAMP2 Abcam Cat# 25631 Anti-Capthesin B Cell Signaling Technology Cat#31718; RRID: AB_2687580 Anti-Capthesin D Abcam Cat# ab198326 Anti-Rab 11 Cell Signaling Technology Cat# 5589; RRID: AB_10693925 Anti-Rab 8b Thermo Fisher Scientific CAT# PA5-67354; RRID: AB_2664303 Anti-Flag-HRP Sigma Aldrich Cat# A8592; RRID: AB_439702 Anti-HA-HRP Abcam Cat# ab128131; RRID: AB_11143947 Anti-c-Myc Roche Cat# 11667203001; RRID: AB_390911 Bacterial and Virus Strains A/California/07/2009(H1N1 pandemic) Jahan et al.9 N/A A/Oklahoma/309/06 (H3N2) Fan et al.43 N/A A/Hong Kong/1073/99 (H9N2) Chan et al.44 N/A A/Vietnam/1203/2004 (H5N1) Herfst et al.45 N/A A/Shanghai/02/2013 (H7N9) Mok et al.46 N/A Human coronavirus 229E ATCC VR-740 BetaCoV/Hong Kong/VM20001061/2020 Lv et al.47 N/A ZIKV (NC-14-5132) Lan et al.16 and Li et al.17 N/A Chemocompetent E. coli DH5a Thermo Fisher Scientific Cat# 18265017 (Continued on next page) e1 Developmental Cell 58, 2495–2509.e1–e6, November 20, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins DMEM Thermo Fisher Scientific Cat# 10569010 HEPES Thermo Fisher Scientific Cat# 15630130 Trypsin-EDTA Thermo Fisher Scientific Cat# 25200072 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122 Fetal Bovine Serum (FBS) Thermo Fisher Scientific Cat# 10500064 Phosphate-buffered saline (PBS) Thermo Fisher Scientific Cat# 14040133 Puromycin InvivoGen Cat# ant-pr-1 Hygromycin B Thermo Fisher Scientific Cat# 10687010 Triton X-100 Sigma Aldrich Cat# 93443 Complete protease inhibitor cocktail Roche Cat# 11836145001 Normal Goat Serum (NGS) Thermo Fisher Scientific Cat# 31873 MG132 Sigma Aldrich Cat# M7449 TransITLT1 transfection reagent Mirus Cat# MIR2304 Lipofectamine 3000 Thermo Fisher Scientific Cat# L3000008 Polyethylenimine (PEI) Polysciences Cat# 23966-1 DharmaFECT1 Dharmacon Cat# T-2001-03 Octadecyl Rhodamine B Chloride (R18) Thermo Fisher Scientific Cat# O246 Fix Perm Kits BD PharMingen Cat# 554714 TPCK-Trypisn Thermo Fisher Scientific Cat# 20233 T4 ligase New England Biolabs Cat# M0202S Endo H New England Biolabs Cat# P0702S PNGase F New England Biolabs Cat# P0704S Critical Commercial Assays TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.USP28.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.SMURF1.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.BECN1.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.MAP1LC3B.13 PrimeScript RT Reagent Kit TAKARA Cat# RR037A MiniBEST Universal RNA Extraction Kit TAKARA Cat# 9767 MiniBEST Viral RNA Extraction Kit TAKARA Cat# 9766 TB Green Premix Ex Taq II (Tli RNase H Plus) TAKARA Cat# RR820A LEGENDplex Human Anti-Virus Response Panel Biolegend Cat# 740390 LEGENDplex Human Proinflammatory Chemokine Panel 1 Biolegend Cat# 740984 Deposited Data Proteomics; PRIDE database This study Accession: PXD034797 Experimental Models: Cell Lines ..

    Saline:

    Article Title: Usp25-Erlin1/2 activity limits cholesterol flux to restrict virus infection
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Usp25 Abcam Cat# ab187156 Anti-SMURF1 Santa Cruz Cat# sc-100616 Anti-Usp28 Abcam Cat# ab126604; RRID: AB_11127442 Anti-GAPDH Abcam Cat# ab8245; RRID: AB_2107448 Anti-PB2 Thermo Fisher Scientific Cat# PA5-32220; RRID: AB_2549693 Anti-HA Abcam Cat# ab119966; RRID: AB_10899358 Anti-NS1 Santa Cruz Cat# sc-130568; RRID: AB_2011757 Anti-NP Abcam Cat# ab128193; RRID: AB_11143769 Anti-RIG-I Abcam Cat# ab180675 Anti-MAVS Cell Signaling Technology Cat# 3993; RRID: AB_823565 Anti-TBK1 Cell Signaling Technology Cat# 3504; RRID: AB_2255663 Anti-Phospho-TBK1 (Ser172) Cell Signaling Technology Cat# 5483; RRID: AB_10693472 TRAF3 Abcam Cat# 36988 Anti-IRF3 Cell Signaling Technology Cat# 4302; RRID: AB_1904036 Anti-Phospho-IRF3 (Ser386) Cell Signaling Technology Cat# 37829; RRID: AB_2799121 Anti-IRF7 Cell Signaling Technology Cat# 4920; RRID: AB_2127551 Anti-STAT1 Cell Signaling Technology Cat# 9172; RRID: AB_2198300 Anti-Phospho-STAT1 (Tyr701) Cell Signaling Technology Cat# 9167; RRID: AB_561284 Anti-STAT2 Cell Signaling Technology Cat# 4594; RRID: AB_2271323 IFIT1 Abcam Cat# 236256 Anti-Phospho-STAT2 (Tyr690) Cell Signaling Technology Cat# 88410; RRID: AB_2800123 Anti-Beclin 1 Cell Signaling Technology Cat# 3495; RRID: AB_1903911 Anti-ATG 5 Cell Signaling Technology Cat 12994; RRID: AB_2630393 Anti-ATG 12 Cell Signaling Technology Cat# 4180; RRID: AB_1903898 Anti-LC 3 Cell Signaling Technology Cat# 2775; RRID: AB_915950 Anti-LAMP1 Cell Signaling Technology Cat# 9091; RRID: AB_2687579 Anti-LAMP2 Abcam Cat# 25631 Anti-Capthesin B Cell Signaling Technology Cat#31718; RRID: AB_2687580 Anti-Capthesin D Abcam Cat# ab198326 Anti-Rab 11 Cell Signaling Technology Cat# 5589; RRID: AB_10693925 Anti-Rab 8b Thermo Fisher Scientific CAT# PA5-67354; RRID: AB_2664303 Anti-Flag-HRP Sigma Aldrich Cat# A8592; RRID: AB_439702 Anti-HA-HRP Abcam Cat# ab128131; RRID: AB_11143947 Anti-c-Myc Roche Cat# 11667203001; RRID: AB_390911 Bacterial and Virus Strains A/California/07/2009(H1N1 pandemic) Jahan et al.9 N/A A/Oklahoma/309/06 (H3N2) Fan et al.43 N/A A/Hong Kong/1073/99 (H9N2) Chan et al.44 N/A A/Vietnam/1203/2004 (H5N1) Herfst et al.45 N/A A/Shanghai/02/2013 (H7N9) Mok et al.46 N/A Human coronavirus 229E ATCC VR-740 BetaCoV/Hong Kong/VM20001061/2020 Lv et al.47 N/A ZIKV (NC-14-5132) Lan et al.16 and Li et al.17 N/A Chemocompetent E. coli DH5a Thermo Fisher Scientific Cat# 18265017 (Continued on next page) e1 Developmental Cell 58, 2495–2509.e1–e6, November 20, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins DMEM Thermo Fisher Scientific Cat# 10569010 HEPES Thermo Fisher Scientific Cat# 15630130 Trypsin-EDTA Thermo Fisher Scientific Cat# 25200072 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122 Fetal Bovine Serum (FBS) Thermo Fisher Scientific Cat# 10500064 Phosphate-buffered saline (PBS) Thermo Fisher Scientific Cat# 14040133 Puromycin InvivoGen Cat# ant-pr-1 Hygromycin B Thermo Fisher Scientific Cat# 10687010 Triton X-100 Sigma Aldrich Cat# 93443 Complete protease inhibitor cocktail Roche Cat# 11836145001 Normal Goat Serum (NGS) Thermo Fisher Scientific Cat# 31873 MG132 Sigma Aldrich Cat# M7449 TransITLT1 transfection reagent Mirus Cat# MIR2304 Lipofectamine 3000 Thermo Fisher Scientific Cat# L3000008 Polyethylenimine (PEI) Polysciences Cat# 23966-1 DharmaFECT1 Dharmacon Cat# T-2001-03 Octadecyl Rhodamine B Chloride (R18) Thermo Fisher Scientific Cat# O246 Fix Perm Kits BD PharMingen Cat# 554714 TPCK-Trypisn Thermo Fisher Scientific Cat# 20233 T4 ligase New England Biolabs Cat# M0202S Endo H New England Biolabs Cat# P0702S PNGase F New England Biolabs Cat# P0704S Critical Commercial Assays TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.USP28.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.SMURF1.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.BECN1.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.MAP1LC3B.13 PrimeScript RT Reagent Kit TAKARA Cat# RR037A MiniBEST Universal RNA Extraction Kit TAKARA Cat# 9767 MiniBEST Viral RNA Extraction Kit TAKARA Cat# 9766 TB Green Premix Ex Taq II (Tli RNase H Plus) TAKARA Cat# RR820A LEGENDplex Human Anti-Virus Response Panel Biolegend Cat# 740390 LEGENDplex Human Proinflammatory Chemokine Panel 1 Biolegend Cat# 740984 Deposited Data Proteomics; PRIDE database This study Accession: PXD034797 Experimental Models: Cell Lines ..

    Article Title: Usp25-Erlin1/2 activity limits cholesterol flux to restrict virus infection.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Usp25 Abcam Cat# ab187156 Anti-SMURF1 Santa Cruz Cat# sc-100616 Anti-Usp28 Abcam Cat# ab126604; RRID: AB_11127442 Anti-GAPDH Abcam Cat# ab8245; RRID: AB_2107448 Anti-PB2 Thermo Fisher Scientific Cat# PA5-32220; RRID: AB_2549693 Anti-HA Abcam Cat# ab119966; RRID: AB_10899358 Anti-NS1 Santa Cruz Cat# sc-130568; RRID: AB_2011757 Anti-NP Abcam Cat# ab128193; RRID: AB_11143769 Anti-RIG-I Abcam Cat# ab180675 Anti-MAVS Cell Signaling Technology Cat# 3993; RRID: AB_823565 Anti-TBK1 Cell Signaling Technology Cat# 3504; RRID: AB_2255663 Anti-Phospho-TBK1 (Ser172) Cell Signaling Technology Cat# 5483; RRID: AB_10693472 TRAF3 Abcam Cat# 36988 Anti-IRF3 Cell Signaling Technology Cat# 4302; RRID: AB_1904036 Anti-Phospho-IRF3 (Ser386) Cell Signaling Technology Cat# 37829; RRID: AB_2799121 Anti-IRF7 Cell Signaling Technology Cat# 4920; RRID: AB_2127551 Anti-STAT1 Cell Signaling Technology Cat# 9172; RRID: AB_2198300 Anti-Phospho-STAT1 (Tyr701) Cell Signaling Technology Cat# 9167; RRID: AB_561284 Anti-STAT2 Cell Signaling Technology Cat# 4594; RRID: AB_2271323 IFIT1 Abcam Cat# 236256 Anti-Phospho-STAT2 (Tyr690) Cell Signaling Technology Cat# 88410; RRID: AB_2800123 Anti-Beclin 1 Cell Signaling Technology Cat# 3495; RRID: AB_1903911 Anti-ATG 5 Cell Signaling Technology Cat 12994; RRID: AB_2630393 Anti-ATG 12 Cell Signaling Technology Cat# 4180; RRID: AB_1903898 Anti-LC 3 Cell Signaling Technology Cat# 2775; RRID: AB_915950 Anti-LAMP1 Cell Signaling Technology Cat# 9091; RRID: AB_2687579 Anti-LAMP2 Abcam Cat# 25631 Anti-Capthesin B Cell Signaling Technology Cat#31718; RRID: AB_2687580 Anti-Capthesin D Abcam Cat# ab198326 Anti-Rab 11 Cell Signaling Technology Cat# 5589; RRID: AB_10693925 Anti-Rab 8b Thermo Fisher Scientific CAT# PA5-67354; RRID: AB_2664303 Anti-Flag-HRP Sigma Aldrich Cat# A8592; RRID: AB_439702 Anti-HA-HRP Abcam Cat# ab128131; RRID: AB_11143947 Anti-c-Myc Roche Cat# 11667203001; RRID: AB_390911 Bacterial and Virus Strains A/California/07/2009(H1N1 pandemic) Jahan et al.9 N/A A/Oklahoma/309/06 (H3N2) Fan et al.43 N/A A/Hong Kong/1073/99 (H9N2) Chan et al.44 N/A A/Vietnam/1203/2004 (H5N1) Herfst et al.45 N/A A/Shanghai/02/2013 (H7N9) Mok et al.46 N/A Human coronavirus 229E ATCC VR-740 BetaCoV/Hong Kong/VM20001061/2020 Lv et al.47 N/A ZIKV (NC-14-5132) Lan et al.16 and Li et al.17 N/A Chemocompetent E. coli DH5a Thermo Fisher Scientific Cat# 18265017 (Continued on next page) e1 Developmental Cell 58, 2495–2509.e1–e6, November 20, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins DMEM Thermo Fisher Scientific Cat# 10569010 HEPES Thermo Fisher Scientific Cat# 15630130 Trypsin-EDTA Thermo Fisher Scientific Cat# 25200072 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122 Fetal Bovine Serum (FBS) Thermo Fisher Scientific Cat# 10500064 Phosphate-buffered saline (PBS) Thermo Fisher Scientific Cat# 14040133 Puromycin InvivoGen Cat# ant-pr-1 Hygromycin B Thermo Fisher Scientific Cat# 10687010 Triton X-100 Sigma Aldrich Cat# 93443 Complete protease inhibitor cocktail Roche Cat# 11836145001 Normal Goat Serum (NGS) Thermo Fisher Scientific Cat# 31873 MG132 Sigma Aldrich Cat# M7449 TransITLT1 transfection reagent Mirus Cat# MIR2304 Lipofectamine 3000 Thermo Fisher Scientific Cat# L3000008 Polyethylenimine (PEI) Polysciences Cat# 23966-1 DharmaFECT1 Dharmacon Cat# T-2001-03 Octadecyl Rhodamine B Chloride (R18) Thermo Fisher Scientific Cat# O246 Fix Perm Kits BD PharMingen Cat# 554714 TPCK-Trypisn Thermo Fisher Scientific Cat# 20233 T4 ligase New England Biolabs Cat# M0202S Endo H New England Biolabs Cat# P0702S PNGase F New England Biolabs Cat# P0704S Critical Commercial Assays TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.USP28.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.SMURF1.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.BECN1.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.MAP1LC3B.13 PrimeScript RT Reagent Kit TAKARA Cat# RR037A MiniBEST Universal RNA Extraction Kit TAKARA Cat# 9767 MiniBEST Viral RNA Extraction Kit TAKARA Cat# 9766 TB Green Premix Ex Taq II (Tli RNase H Plus) TAKARA Cat# RR820A LEGENDplex Human Anti-Virus Response Panel Biolegend Cat# 740390 LEGENDplex Human Proinflammatory Chemokine Panel 1 Biolegend Cat# 740984 Deposited Data Proteomics; PRIDE database This study Accession: PXD034797 Experimental Models: Cell Lines ..

    Protease Inhibitor:

    Article Title: Usp25-Erlin1/2 activity limits cholesterol flux to restrict virus infection
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Usp25 Abcam Cat# ab187156 Anti-SMURF1 Santa Cruz Cat# sc-100616 Anti-Usp28 Abcam Cat# ab126604; RRID: AB_11127442 Anti-GAPDH Abcam Cat# ab8245; RRID: AB_2107448 Anti-PB2 Thermo Fisher Scientific Cat# PA5-32220; RRID: AB_2549693 Anti-HA Abcam Cat# ab119966; RRID: AB_10899358 Anti-NS1 Santa Cruz Cat# sc-130568; RRID: AB_2011757 Anti-NP Abcam Cat# ab128193; RRID: AB_11143769 Anti-RIG-I Abcam Cat# ab180675 Anti-MAVS Cell Signaling Technology Cat# 3993; RRID: AB_823565 Anti-TBK1 Cell Signaling Technology Cat# 3504; RRID: AB_2255663 Anti-Phospho-TBK1 (Ser172) Cell Signaling Technology Cat# 5483; RRID: AB_10693472 TRAF3 Abcam Cat# 36988 Anti-IRF3 Cell Signaling Technology Cat# 4302; RRID: AB_1904036 Anti-Phospho-IRF3 (Ser386) Cell Signaling Technology Cat# 37829; RRID: AB_2799121 Anti-IRF7 Cell Signaling Technology Cat# 4920; RRID: AB_2127551 Anti-STAT1 Cell Signaling Technology Cat# 9172; RRID: AB_2198300 Anti-Phospho-STAT1 (Tyr701) Cell Signaling Technology Cat# 9167; RRID: AB_561284 Anti-STAT2 Cell Signaling Technology Cat# 4594; RRID: AB_2271323 IFIT1 Abcam Cat# 236256 Anti-Phospho-STAT2 (Tyr690) Cell Signaling Technology Cat# 88410; RRID: AB_2800123 Anti-Beclin 1 Cell Signaling Technology Cat# 3495; RRID: AB_1903911 Anti-ATG 5 Cell Signaling Technology Cat 12994; RRID: AB_2630393 Anti-ATG 12 Cell Signaling Technology Cat# 4180; RRID: AB_1903898 Anti-LC 3 Cell Signaling Technology Cat# 2775; RRID: AB_915950 Anti-LAMP1 Cell Signaling Technology Cat# 9091; RRID: AB_2687579 Anti-LAMP2 Abcam Cat# 25631 Anti-Capthesin B Cell Signaling Technology Cat#31718; RRID: AB_2687580 Anti-Capthesin D Abcam Cat# ab198326 Anti-Rab 11 Cell Signaling Technology Cat# 5589; RRID: AB_10693925 Anti-Rab 8b Thermo Fisher Scientific CAT# PA5-67354; RRID: AB_2664303 Anti-Flag-HRP Sigma Aldrich Cat# A8592; RRID: AB_439702 Anti-HA-HRP Abcam Cat# ab128131; RRID: AB_11143947 Anti-c-Myc Roche Cat# 11667203001; RRID: AB_390911 Bacterial and Virus Strains A/California/07/2009(H1N1 pandemic) Jahan et al.9 N/A A/Oklahoma/309/06 (H3N2) Fan et al.43 N/A A/Hong Kong/1073/99 (H9N2) Chan et al.44 N/A A/Vietnam/1203/2004 (H5N1) Herfst et al.45 N/A A/Shanghai/02/2013 (H7N9) Mok et al.46 N/A Human coronavirus 229E ATCC VR-740 BetaCoV/Hong Kong/VM20001061/2020 Lv et al.47 N/A ZIKV (NC-14-5132) Lan et al.16 and Li et al.17 N/A Chemocompetent E. coli DH5a Thermo Fisher Scientific Cat# 18265017 (Continued on next page) e1 Developmental Cell 58, 2495–2509.e1–e6, November 20, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins DMEM Thermo Fisher Scientific Cat# 10569010 HEPES Thermo Fisher Scientific Cat# 15630130 Trypsin-EDTA Thermo Fisher Scientific Cat# 25200072 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122 Fetal Bovine Serum (FBS) Thermo Fisher Scientific Cat# 10500064 Phosphate-buffered saline (PBS) Thermo Fisher Scientific Cat# 14040133 Puromycin InvivoGen Cat# ant-pr-1 Hygromycin B Thermo Fisher Scientific Cat# 10687010 Triton X-100 Sigma Aldrich Cat# 93443 Complete protease inhibitor cocktail Roche Cat# 11836145001 Normal Goat Serum (NGS) Thermo Fisher Scientific Cat# 31873 MG132 Sigma Aldrich Cat# M7449 TransITLT1 transfection reagent Mirus Cat# MIR2304 Lipofectamine 3000 Thermo Fisher Scientific Cat# L3000008 Polyethylenimine (PEI) Polysciences Cat# 23966-1 DharmaFECT1 Dharmacon Cat# T-2001-03 Octadecyl Rhodamine B Chloride (R18) Thermo Fisher Scientific Cat# O246 Fix Perm Kits BD PharMingen Cat# 554714 TPCK-Trypisn Thermo Fisher Scientific Cat# 20233 T4 ligase New England Biolabs Cat# M0202S Endo H New England Biolabs Cat# P0702S PNGase F New England Biolabs Cat# P0704S Critical Commercial Assays TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.USP28.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.SMURF1.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.BECN1.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.MAP1LC3B.13 PrimeScript RT Reagent Kit TAKARA Cat# RR037A MiniBEST Universal RNA Extraction Kit TAKARA Cat# 9767 MiniBEST Viral RNA Extraction Kit TAKARA Cat# 9766 TB Green Premix Ex Taq II (Tli RNase H Plus) TAKARA Cat# RR820A LEGENDplex Human Anti-Virus Response Panel Biolegend Cat# 740390 LEGENDplex Human Proinflammatory Chemokine Panel 1 Biolegend Cat# 740984 Deposited Data Proteomics; PRIDE database This study Accession: PXD034797 Experimental Models: Cell Lines ..

    Article Title: Usp25-Erlin1/2 activity limits cholesterol flux to restrict virus infection.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Usp25 Abcam Cat# ab187156 Anti-SMURF1 Santa Cruz Cat# sc-100616 Anti-Usp28 Abcam Cat# ab126604; RRID: AB_11127442 Anti-GAPDH Abcam Cat# ab8245; RRID: AB_2107448 Anti-PB2 Thermo Fisher Scientific Cat# PA5-32220; RRID: AB_2549693 Anti-HA Abcam Cat# ab119966; RRID: AB_10899358 Anti-NS1 Santa Cruz Cat# sc-130568; RRID: AB_2011757 Anti-NP Abcam Cat# ab128193; RRID: AB_11143769 Anti-RIG-I Abcam Cat# ab180675 Anti-MAVS Cell Signaling Technology Cat# 3993; RRID: AB_823565 Anti-TBK1 Cell Signaling Technology Cat# 3504; RRID: AB_2255663 Anti-Phospho-TBK1 (Ser172) Cell Signaling Technology Cat# 5483; RRID: AB_10693472 TRAF3 Abcam Cat# 36988 Anti-IRF3 Cell Signaling Technology Cat# 4302; RRID: AB_1904036 Anti-Phospho-IRF3 (Ser386) Cell Signaling Technology Cat# 37829; RRID: AB_2799121 Anti-IRF7 Cell Signaling Technology Cat# 4920; RRID: AB_2127551 Anti-STAT1 Cell Signaling Technology Cat# 9172; RRID: AB_2198300 Anti-Phospho-STAT1 (Tyr701) Cell Signaling Technology Cat# 9167; RRID: AB_561284 Anti-STAT2 Cell Signaling Technology Cat# 4594; RRID: AB_2271323 IFIT1 Abcam Cat# 236256 Anti-Phospho-STAT2 (Tyr690) Cell Signaling Technology Cat# 88410; RRID: AB_2800123 Anti-Beclin 1 Cell Signaling Technology Cat# 3495; RRID: AB_1903911 Anti-ATG 5 Cell Signaling Technology Cat 12994; RRID: AB_2630393 Anti-ATG 12 Cell Signaling Technology Cat# 4180; RRID: AB_1903898 Anti-LC 3 Cell Signaling Technology Cat# 2775; RRID: AB_915950 Anti-LAMP1 Cell Signaling Technology Cat# 9091; RRID: AB_2687579 Anti-LAMP2 Abcam Cat# 25631 Anti-Capthesin B Cell Signaling Technology Cat#31718; RRID: AB_2687580 Anti-Capthesin D Abcam Cat# ab198326 Anti-Rab 11 Cell Signaling Technology Cat# 5589; RRID: AB_10693925 Anti-Rab 8b Thermo Fisher Scientific CAT# PA5-67354; RRID: AB_2664303 Anti-Flag-HRP Sigma Aldrich Cat# A8592; RRID: AB_439702 Anti-HA-HRP Abcam Cat# ab128131; RRID: AB_11143947 Anti-c-Myc Roche Cat# 11667203001; RRID: AB_390911 Bacterial and Virus Strains A/California/07/2009(H1N1 pandemic) Jahan et al.9 N/A A/Oklahoma/309/06 (H3N2) Fan et al.43 N/A A/Hong Kong/1073/99 (H9N2) Chan et al.44 N/A A/Vietnam/1203/2004 (H5N1) Herfst et al.45 N/A A/Shanghai/02/2013 (H7N9) Mok et al.46 N/A Human coronavirus 229E ATCC VR-740 BetaCoV/Hong Kong/VM20001061/2020 Lv et al.47 N/A ZIKV (NC-14-5132) Lan et al.16 and Li et al.17 N/A Chemocompetent E. coli DH5a Thermo Fisher Scientific Cat# 18265017 (Continued on next page) e1 Developmental Cell 58, 2495–2509.e1–e6, November 20, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins DMEM Thermo Fisher Scientific Cat# 10569010 HEPES Thermo Fisher Scientific Cat# 15630130 Trypsin-EDTA Thermo Fisher Scientific Cat# 25200072 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122 Fetal Bovine Serum (FBS) Thermo Fisher Scientific Cat# 10500064 Phosphate-buffered saline (PBS) Thermo Fisher Scientific Cat# 14040133 Puromycin InvivoGen Cat# ant-pr-1 Hygromycin B Thermo Fisher Scientific Cat# 10687010 Triton X-100 Sigma Aldrich Cat# 93443 Complete protease inhibitor cocktail Roche Cat# 11836145001 Normal Goat Serum (NGS) Thermo Fisher Scientific Cat# 31873 MG132 Sigma Aldrich Cat# M7449 TransITLT1 transfection reagent Mirus Cat# MIR2304 Lipofectamine 3000 Thermo Fisher Scientific Cat# L3000008 Polyethylenimine (PEI) Polysciences Cat# 23966-1 DharmaFECT1 Dharmacon Cat# T-2001-03 Octadecyl Rhodamine B Chloride (R18) Thermo Fisher Scientific Cat# O246 Fix Perm Kits BD PharMingen Cat# 554714 TPCK-Trypisn Thermo Fisher Scientific Cat# 20233 T4 ligase New England Biolabs Cat# M0202S Endo H New England Biolabs Cat# P0702S PNGase F New England Biolabs Cat# P0704S Critical Commercial Assays TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.USP28.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.SMURF1.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.BECN1.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.MAP1LC3B.13 PrimeScript RT Reagent Kit TAKARA Cat# RR037A MiniBEST Universal RNA Extraction Kit TAKARA Cat# 9767 MiniBEST Viral RNA Extraction Kit TAKARA Cat# 9766 TB Green Premix Ex Taq II (Tli RNase H Plus) TAKARA Cat# RR820A LEGENDplex Human Anti-Virus Response Panel Biolegend Cat# 740390 LEGENDplex Human Proinflammatory Chemokine Panel 1 Biolegend Cat# 740984 Deposited Data Proteomics; PRIDE database This study Accession: PXD034797 Experimental Models: Cell Lines ..

    Next-Generation Sequencing:

    Article Title: Usp25-Erlin1/2 activity limits cholesterol flux to restrict virus infection
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Usp25 Abcam Cat# ab187156 Anti-SMURF1 Santa Cruz Cat# sc-100616 Anti-Usp28 Abcam Cat# ab126604; RRID: AB_11127442 Anti-GAPDH Abcam Cat# ab8245; RRID: AB_2107448 Anti-PB2 Thermo Fisher Scientific Cat# PA5-32220; RRID: AB_2549693 Anti-HA Abcam Cat# ab119966; RRID: AB_10899358 Anti-NS1 Santa Cruz Cat# sc-130568; RRID: AB_2011757 Anti-NP Abcam Cat# ab128193; RRID: AB_11143769 Anti-RIG-I Abcam Cat# ab180675 Anti-MAVS Cell Signaling Technology Cat# 3993; RRID: AB_823565 Anti-TBK1 Cell Signaling Technology Cat# 3504; RRID: AB_2255663 Anti-Phospho-TBK1 (Ser172) Cell Signaling Technology Cat# 5483; RRID: AB_10693472 TRAF3 Abcam Cat# 36988 Anti-IRF3 Cell Signaling Technology Cat# 4302; RRID: AB_1904036 Anti-Phospho-IRF3 (Ser386) Cell Signaling Technology Cat# 37829; RRID: AB_2799121 Anti-IRF7 Cell Signaling Technology Cat# 4920; RRID: AB_2127551 Anti-STAT1 Cell Signaling Technology Cat# 9172; RRID: AB_2198300 Anti-Phospho-STAT1 (Tyr701) Cell Signaling Technology Cat# 9167; RRID: AB_561284 Anti-STAT2 Cell Signaling Technology Cat# 4594; RRID: AB_2271323 IFIT1 Abcam Cat# 236256 Anti-Phospho-STAT2 (Tyr690) Cell Signaling Technology Cat# 88410; RRID: AB_2800123 Anti-Beclin 1 Cell Signaling Technology Cat# 3495; RRID: AB_1903911 Anti-ATG 5 Cell Signaling Technology Cat 12994; RRID: AB_2630393 Anti-ATG 12 Cell Signaling Technology Cat# 4180; RRID: AB_1903898 Anti-LC 3 Cell Signaling Technology Cat# 2775; RRID: AB_915950 Anti-LAMP1 Cell Signaling Technology Cat# 9091; RRID: AB_2687579 Anti-LAMP2 Abcam Cat# 25631 Anti-Capthesin B Cell Signaling Technology Cat#31718; RRID: AB_2687580 Anti-Capthesin D Abcam Cat# ab198326 Anti-Rab 11 Cell Signaling Technology Cat# 5589; RRID: AB_10693925 Anti-Rab 8b Thermo Fisher Scientific CAT# PA5-67354; RRID: AB_2664303 Anti-Flag-HRP Sigma Aldrich Cat# A8592; RRID: AB_439702 Anti-HA-HRP Abcam Cat# ab128131; RRID: AB_11143947 Anti-c-Myc Roche Cat# 11667203001; RRID: AB_390911 Bacterial and Virus Strains A/California/07/2009(H1N1 pandemic) Jahan et al.9 N/A A/Oklahoma/309/06 (H3N2) Fan et al.43 N/A A/Hong Kong/1073/99 (H9N2) Chan et al.44 N/A A/Vietnam/1203/2004 (H5N1) Herfst et al.45 N/A A/Shanghai/02/2013 (H7N9) Mok et al.46 N/A Human coronavirus 229E ATCC VR-740 BetaCoV/Hong Kong/VM20001061/2020 Lv et al.47 N/A ZIKV (NC-14-5132) Lan et al.16 and Li et al.17 N/A Chemocompetent E. coli DH5a Thermo Fisher Scientific Cat# 18265017 (Continued on next page) e1 Developmental Cell 58, 2495–2509.e1–e6, November 20, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins DMEM Thermo Fisher Scientific Cat# 10569010 HEPES Thermo Fisher Scientific Cat# 15630130 Trypsin-EDTA Thermo Fisher Scientific Cat# 25200072 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122 Fetal Bovine Serum (FBS) Thermo Fisher Scientific Cat# 10500064 Phosphate-buffered saline (PBS) Thermo Fisher Scientific Cat# 14040133 Puromycin InvivoGen Cat# ant-pr-1 Hygromycin B Thermo Fisher Scientific Cat# 10687010 Triton X-100 Sigma Aldrich Cat# 93443 Complete protease inhibitor cocktail Roche Cat# 11836145001 Normal Goat Serum (NGS) Thermo Fisher Scientific Cat# 31873 MG132 Sigma Aldrich Cat# M7449 TransITLT1 transfection reagent Mirus Cat# MIR2304 Lipofectamine 3000 Thermo Fisher Scientific Cat# L3000008 Polyethylenimine (PEI) Polysciences Cat# 23966-1 DharmaFECT1 Dharmacon Cat# T-2001-03 Octadecyl Rhodamine B Chloride (R18) Thermo Fisher Scientific Cat# O246 Fix Perm Kits BD PharMingen Cat# 554714 TPCK-Trypisn Thermo Fisher Scientific Cat# 20233 T4 ligase New England Biolabs Cat# M0202S Endo H New England Biolabs Cat# P0702S PNGase F New England Biolabs Cat# P0704S Critical Commercial Assays TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.USP28.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.SMURF1.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.BECN1.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.MAP1LC3B.13 PrimeScript RT Reagent Kit TAKARA Cat# RR037A MiniBEST Universal RNA Extraction Kit TAKARA Cat# 9767 MiniBEST Viral RNA Extraction Kit TAKARA Cat# 9766 TB Green Premix Ex Taq II (Tli RNase H Plus) TAKARA Cat# RR820A LEGENDplex Human Anti-Virus Response Panel Biolegend Cat# 740390 LEGENDplex Human Proinflammatory Chemokine Panel 1 Biolegend Cat# 740984 Deposited Data Proteomics; PRIDE database This study Accession: PXD034797 Experimental Models: Cell Lines ..

    Article Title: Usp25-Erlin1/2 activity limits cholesterol flux to restrict virus infection.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Usp25 Abcam Cat# ab187156 Anti-SMURF1 Santa Cruz Cat# sc-100616 Anti-Usp28 Abcam Cat# ab126604; RRID: AB_11127442 Anti-GAPDH Abcam Cat# ab8245; RRID: AB_2107448 Anti-PB2 Thermo Fisher Scientific Cat# PA5-32220; RRID: AB_2549693 Anti-HA Abcam Cat# ab119966; RRID: AB_10899358 Anti-NS1 Santa Cruz Cat# sc-130568; RRID: AB_2011757 Anti-NP Abcam Cat# ab128193; RRID: AB_11143769 Anti-RIG-I Abcam Cat# ab180675 Anti-MAVS Cell Signaling Technology Cat# 3993; RRID: AB_823565 Anti-TBK1 Cell Signaling Technology Cat# 3504; RRID: AB_2255663 Anti-Phospho-TBK1 (Ser172) Cell Signaling Technology Cat# 5483; RRID: AB_10693472 TRAF3 Abcam Cat# 36988 Anti-IRF3 Cell Signaling Technology Cat# 4302; RRID: AB_1904036 Anti-Phospho-IRF3 (Ser386) Cell Signaling Technology Cat# 37829; RRID: AB_2799121 Anti-IRF7 Cell Signaling Technology Cat# 4920; RRID: AB_2127551 Anti-STAT1 Cell Signaling Technology Cat# 9172; RRID: AB_2198300 Anti-Phospho-STAT1 (Tyr701) Cell Signaling Technology Cat# 9167; RRID: AB_561284 Anti-STAT2 Cell Signaling Technology Cat# 4594; RRID: AB_2271323 IFIT1 Abcam Cat# 236256 Anti-Phospho-STAT2 (Tyr690) Cell Signaling Technology Cat# 88410; RRID: AB_2800123 Anti-Beclin 1 Cell Signaling Technology Cat# 3495; RRID: AB_1903911 Anti-ATG 5 Cell Signaling Technology Cat 12994; RRID: AB_2630393 Anti-ATG 12 Cell Signaling Technology Cat# 4180; RRID: AB_1903898 Anti-LC 3 Cell Signaling Technology Cat# 2775; RRID: AB_915950 Anti-LAMP1 Cell Signaling Technology Cat# 9091; RRID: AB_2687579 Anti-LAMP2 Abcam Cat# 25631 Anti-Capthesin B Cell Signaling Technology Cat#31718; RRID: AB_2687580 Anti-Capthesin D Abcam Cat# ab198326 Anti-Rab 11 Cell Signaling Technology Cat# 5589; RRID: AB_10693925 Anti-Rab 8b Thermo Fisher Scientific CAT# PA5-67354; RRID: AB_2664303 Anti-Flag-HRP Sigma Aldrich Cat# A8592; RRID: AB_439702 Anti-HA-HRP Abcam Cat# ab128131; RRID: AB_11143947 Anti-c-Myc Roche Cat# 11667203001; RRID: AB_390911 Bacterial and Virus Strains A/California/07/2009(H1N1 pandemic) Jahan et al.9 N/A A/Oklahoma/309/06 (H3N2) Fan et al.43 N/A A/Hong Kong/1073/99 (H9N2) Chan et al.44 N/A A/Vietnam/1203/2004 (H5N1) Herfst et al.45 N/A A/Shanghai/02/2013 (H7N9) Mok et al.46 N/A Human coronavirus 229E ATCC VR-740 BetaCoV/Hong Kong/VM20001061/2020 Lv et al.47 N/A ZIKV (NC-14-5132) Lan et al.16 and Li et al.17 N/A Chemocompetent E. coli DH5a Thermo Fisher Scientific Cat# 18265017 (Continued on next page) e1 Developmental Cell 58, 2495–2509.e1–e6, November 20, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins DMEM Thermo Fisher Scientific Cat# 10569010 HEPES Thermo Fisher Scientific Cat# 15630130 Trypsin-EDTA Thermo Fisher Scientific Cat# 25200072 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122 Fetal Bovine Serum (FBS) Thermo Fisher Scientific Cat# 10500064 Phosphate-buffered saline (PBS) Thermo Fisher Scientific Cat# 14040133 Puromycin InvivoGen Cat# ant-pr-1 Hygromycin B Thermo Fisher Scientific Cat# 10687010 Triton X-100 Sigma Aldrich Cat# 93443 Complete protease inhibitor cocktail Roche Cat# 11836145001 Normal Goat Serum (NGS) Thermo Fisher Scientific Cat# 31873 MG132 Sigma Aldrich Cat# M7449 TransITLT1 transfection reagent Mirus Cat# MIR2304 Lipofectamine 3000 Thermo Fisher Scientific Cat# L3000008 Polyethylenimine (PEI) Polysciences Cat# 23966-1 DharmaFECT1 Dharmacon Cat# T-2001-03 Octadecyl Rhodamine B Chloride (R18) Thermo Fisher Scientific Cat# O246 Fix Perm Kits BD PharMingen Cat# 554714 TPCK-Trypisn Thermo Fisher Scientific Cat# 20233 T4 ligase New England Biolabs Cat# M0202S Endo H New England Biolabs Cat# P0702S PNGase F New England Biolabs Cat# P0704S Critical Commercial Assays TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.USP28.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.SMURF1.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.BECN1.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.MAP1LC3B.13 PrimeScript RT Reagent Kit TAKARA Cat# RR037A MiniBEST Universal RNA Extraction Kit TAKARA Cat# 9767 MiniBEST Viral RNA Extraction Kit TAKARA Cat# 9766 TB Green Premix Ex Taq II (Tli RNase H Plus) TAKARA Cat# RR820A LEGENDplex Human Anti-Virus Response Panel Biolegend Cat# 740390 LEGENDplex Human Proinflammatory Chemokine Panel 1 Biolegend Cat# 740984 Deposited Data Proteomics; PRIDE database This study Accession: PXD034797 Experimental Models: Cell Lines ..

    Transfection:

    Article Title: Usp25-Erlin1/2 activity limits cholesterol flux to restrict virus infection
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Usp25 Abcam Cat# ab187156 Anti-SMURF1 Santa Cruz Cat# sc-100616 Anti-Usp28 Abcam Cat# ab126604; RRID: AB_11127442 Anti-GAPDH Abcam Cat# ab8245; RRID: AB_2107448 Anti-PB2 Thermo Fisher Scientific Cat# PA5-32220; RRID: AB_2549693 Anti-HA Abcam Cat# ab119966; RRID: AB_10899358 Anti-NS1 Santa Cruz Cat# sc-130568; RRID: AB_2011757 Anti-NP Abcam Cat# ab128193; RRID: AB_11143769 Anti-RIG-I Abcam Cat# ab180675 Anti-MAVS Cell Signaling Technology Cat# 3993; RRID: AB_823565 Anti-TBK1 Cell Signaling Technology Cat# 3504; RRID: AB_2255663 Anti-Phospho-TBK1 (Ser172) Cell Signaling Technology Cat# 5483; RRID: AB_10693472 TRAF3 Abcam Cat# 36988 Anti-IRF3 Cell Signaling Technology Cat# 4302; RRID: AB_1904036 Anti-Phospho-IRF3 (Ser386) Cell Signaling Technology Cat# 37829; RRID: AB_2799121 Anti-IRF7 Cell Signaling Technology Cat# 4920; RRID: AB_2127551 Anti-STAT1 Cell Signaling Technology Cat# 9172; RRID: AB_2198300 Anti-Phospho-STAT1 (Tyr701) Cell Signaling Technology Cat# 9167; RRID: AB_561284 Anti-STAT2 Cell Signaling Technology Cat# 4594; RRID: AB_2271323 IFIT1 Abcam Cat# 236256 Anti-Phospho-STAT2 (Tyr690) Cell Signaling Technology Cat# 88410; RRID: AB_2800123 Anti-Beclin 1 Cell Signaling Technology Cat# 3495; RRID: AB_1903911 Anti-ATG 5 Cell Signaling Technology Cat 12994; RRID: AB_2630393 Anti-ATG 12 Cell Signaling Technology Cat# 4180; RRID: AB_1903898 Anti-LC 3 Cell Signaling Technology Cat# 2775; RRID: AB_915950 Anti-LAMP1 Cell Signaling Technology Cat# 9091; RRID: AB_2687579 Anti-LAMP2 Abcam Cat# 25631 Anti-Capthesin B Cell Signaling Technology Cat#31718; RRID: AB_2687580 Anti-Capthesin D Abcam Cat# ab198326 Anti-Rab 11 Cell Signaling Technology Cat# 5589; RRID: AB_10693925 Anti-Rab 8b Thermo Fisher Scientific CAT# PA5-67354; RRID: AB_2664303 Anti-Flag-HRP Sigma Aldrich Cat# A8592; RRID: AB_439702 Anti-HA-HRP Abcam Cat# ab128131; RRID: AB_11143947 Anti-c-Myc Roche Cat# 11667203001; RRID: AB_390911 Bacterial and Virus Strains A/California/07/2009(H1N1 pandemic) Jahan et al.9 N/A A/Oklahoma/309/06 (H3N2) Fan et al.43 N/A A/Hong Kong/1073/99 (H9N2) Chan et al.44 N/A A/Vietnam/1203/2004 (H5N1) Herfst et al.45 N/A A/Shanghai/02/2013 (H7N9) Mok et al.46 N/A Human coronavirus 229E ATCC VR-740 BetaCoV/Hong Kong/VM20001061/2020 Lv et al.47 N/A ZIKV (NC-14-5132) Lan et al.16 and Li et al.17 N/A Chemocompetent E. coli DH5a Thermo Fisher Scientific Cat# 18265017 (Continued on next page) e1 Developmental Cell 58, 2495–2509.e1–e6, November 20, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins DMEM Thermo Fisher Scientific Cat# 10569010 HEPES Thermo Fisher Scientific Cat# 15630130 Trypsin-EDTA Thermo Fisher Scientific Cat# 25200072 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122 Fetal Bovine Serum (FBS) Thermo Fisher Scientific Cat# 10500064 Phosphate-buffered saline (PBS) Thermo Fisher Scientific Cat# 14040133 Puromycin InvivoGen Cat# ant-pr-1 Hygromycin B Thermo Fisher Scientific Cat# 10687010 Triton X-100 Sigma Aldrich Cat# 93443 Complete protease inhibitor cocktail Roche Cat# 11836145001 Normal Goat Serum (NGS) Thermo Fisher Scientific Cat# 31873 MG132 Sigma Aldrich Cat# M7449 TransITLT1 transfection reagent Mirus Cat# MIR2304 Lipofectamine 3000 Thermo Fisher Scientific Cat# L3000008 Polyethylenimine (PEI) Polysciences Cat# 23966-1 DharmaFECT1 Dharmacon Cat# T-2001-03 Octadecyl Rhodamine B Chloride (R18) Thermo Fisher Scientific Cat# O246 Fix Perm Kits BD PharMingen Cat# 554714 TPCK-Trypisn Thermo Fisher Scientific Cat# 20233 T4 ligase New England Biolabs Cat# M0202S Endo H New England Biolabs Cat# P0702S PNGase F New England Biolabs Cat# P0704S Critical Commercial Assays TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.USP28.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.SMURF1.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.BECN1.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.MAP1LC3B.13 PrimeScript RT Reagent Kit TAKARA Cat# RR037A MiniBEST Universal RNA Extraction Kit TAKARA Cat# 9767 MiniBEST Viral RNA Extraction Kit TAKARA Cat# 9766 TB Green Premix Ex Taq II (Tli RNase H Plus) TAKARA Cat# RR820A LEGENDplex Human Anti-Virus Response Panel Biolegend Cat# 740390 LEGENDplex Human Proinflammatory Chemokine Panel 1 Biolegend Cat# 740984 Deposited Data Proteomics; PRIDE database This study Accession: PXD034797 Experimental Models: Cell Lines ..

    Article Title: Usp25-Erlin1/2 activity limits cholesterol flux to restrict virus infection.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Usp25 Abcam Cat# ab187156 Anti-SMURF1 Santa Cruz Cat# sc-100616 Anti-Usp28 Abcam Cat# ab126604; RRID: AB_11127442 Anti-GAPDH Abcam Cat# ab8245; RRID: AB_2107448 Anti-PB2 Thermo Fisher Scientific Cat# PA5-32220; RRID: AB_2549693 Anti-HA Abcam Cat# ab119966; RRID: AB_10899358 Anti-NS1 Santa Cruz Cat# sc-130568; RRID: AB_2011757 Anti-NP Abcam Cat# ab128193; RRID: AB_11143769 Anti-RIG-I Abcam Cat# ab180675 Anti-MAVS Cell Signaling Technology Cat# 3993; RRID: AB_823565 Anti-TBK1 Cell Signaling Technology Cat# 3504; RRID: AB_2255663 Anti-Phospho-TBK1 (Ser172) Cell Signaling Technology Cat# 5483; RRID: AB_10693472 TRAF3 Abcam Cat# 36988 Anti-IRF3 Cell Signaling Technology Cat# 4302; RRID: AB_1904036 Anti-Phospho-IRF3 (Ser386) Cell Signaling Technology Cat# 37829; RRID: AB_2799121 Anti-IRF7 Cell Signaling Technology Cat# 4920; RRID: AB_2127551 Anti-STAT1 Cell Signaling Technology Cat# 9172; RRID: AB_2198300 Anti-Phospho-STAT1 (Tyr701) Cell Signaling Technology Cat# 9167; RRID: AB_561284 Anti-STAT2 Cell Signaling Technology Cat# 4594; RRID: AB_2271323 IFIT1 Abcam Cat# 236256 Anti-Phospho-STAT2 (Tyr690) Cell Signaling Technology Cat# 88410; RRID: AB_2800123 Anti-Beclin 1 Cell Signaling Technology Cat# 3495; RRID: AB_1903911 Anti-ATG 5 Cell Signaling Technology Cat 12994; RRID: AB_2630393 Anti-ATG 12 Cell Signaling Technology Cat# 4180; RRID: AB_1903898 Anti-LC 3 Cell Signaling Technology Cat# 2775; RRID: AB_915950 Anti-LAMP1 Cell Signaling Technology Cat# 9091; RRID: AB_2687579 Anti-LAMP2 Abcam Cat# 25631 Anti-Capthesin B Cell Signaling Technology Cat#31718; RRID: AB_2687580 Anti-Capthesin D Abcam Cat# ab198326 Anti-Rab 11 Cell Signaling Technology Cat# 5589; RRID: AB_10693925 Anti-Rab 8b Thermo Fisher Scientific CAT# PA5-67354; RRID: AB_2664303 Anti-Flag-HRP Sigma Aldrich Cat# A8592; RRID: AB_439702 Anti-HA-HRP Abcam Cat# ab128131; RRID: AB_11143947 Anti-c-Myc Roche Cat# 11667203001; RRID: AB_390911 Bacterial and Virus Strains A/California/07/2009(H1N1 pandemic) Jahan et al.9 N/A A/Oklahoma/309/06 (H3N2) Fan et al.43 N/A A/Hong Kong/1073/99 (H9N2) Chan et al.44 N/A A/Vietnam/1203/2004 (H5N1) Herfst et al.45 N/A A/Shanghai/02/2013 (H7N9) Mok et al.46 N/A Human coronavirus 229E ATCC VR-740 BetaCoV/Hong Kong/VM20001061/2020 Lv et al.47 N/A ZIKV (NC-14-5132) Lan et al.16 and Li et al.17 N/A Chemocompetent E. coli DH5a Thermo Fisher Scientific Cat# 18265017 (Continued on next page) e1 Developmental Cell 58, 2495–2509.e1–e6, November 20, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins DMEM Thermo Fisher Scientific Cat# 10569010 HEPES Thermo Fisher Scientific Cat# 15630130 Trypsin-EDTA Thermo Fisher Scientific Cat# 25200072 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122 Fetal Bovine Serum (FBS) Thermo Fisher Scientific Cat# 10500064 Phosphate-buffered saline (PBS) Thermo Fisher Scientific Cat# 14040133 Puromycin InvivoGen Cat# ant-pr-1 Hygromycin B Thermo Fisher Scientific Cat# 10687010 Triton X-100 Sigma Aldrich Cat# 93443 Complete protease inhibitor cocktail Roche Cat# 11836145001 Normal Goat Serum (NGS) Thermo Fisher Scientific Cat# 31873 MG132 Sigma Aldrich Cat# M7449 TransITLT1 transfection reagent Mirus Cat# MIR2304 Lipofectamine 3000 Thermo Fisher Scientific Cat# L3000008 Polyethylenimine (PEI) Polysciences Cat# 23966-1 DharmaFECT1 Dharmacon Cat# T-2001-03 Octadecyl Rhodamine B Chloride (R18) Thermo Fisher Scientific Cat# O246 Fix Perm Kits BD PharMingen Cat# 554714 TPCK-Trypisn Thermo Fisher Scientific Cat# 20233 T4 ligase New England Biolabs Cat# M0202S Endo H New England Biolabs Cat# P0702S PNGase F New England Biolabs Cat# P0704S Critical Commercial Assays TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.USP28.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.SMURF1.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.BECN1.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.MAP1LC3B.13 PrimeScript RT Reagent Kit TAKARA Cat# RR037A MiniBEST Universal RNA Extraction Kit TAKARA Cat# 9767 MiniBEST Viral RNA Extraction Kit TAKARA Cat# 9766 TB Green Premix Ex Taq II (Tli RNase H Plus) TAKARA Cat# RR820A LEGENDplex Human Anti-Virus Response Panel Biolegend Cat# 740390 LEGENDplex Human Proinflammatory Chemokine Panel 1 Biolegend Cat# 740984 Deposited Data Proteomics; PRIDE database This study Accession: PXD034797 Experimental Models: Cell Lines ..

    RNA Extraction:

    Article Title: Usp25-Erlin1/2 activity limits cholesterol flux to restrict virus infection
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Usp25 Abcam Cat# ab187156 Anti-SMURF1 Santa Cruz Cat# sc-100616 Anti-Usp28 Abcam Cat# ab126604; RRID: AB_11127442 Anti-GAPDH Abcam Cat# ab8245; RRID: AB_2107448 Anti-PB2 Thermo Fisher Scientific Cat# PA5-32220; RRID: AB_2549693 Anti-HA Abcam Cat# ab119966; RRID: AB_10899358 Anti-NS1 Santa Cruz Cat# sc-130568; RRID: AB_2011757 Anti-NP Abcam Cat# ab128193; RRID: AB_11143769 Anti-RIG-I Abcam Cat# ab180675 Anti-MAVS Cell Signaling Technology Cat# 3993; RRID: AB_823565 Anti-TBK1 Cell Signaling Technology Cat# 3504; RRID: AB_2255663 Anti-Phospho-TBK1 (Ser172) Cell Signaling Technology Cat# 5483; RRID: AB_10693472 TRAF3 Abcam Cat# 36988 Anti-IRF3 Cell Signaling Technology Cat# 4302; RRID: AB_1904036 Anti-Phospho-IRF3 (Ser386) Cell Signaling Technology Cat# 37829; RRID: AB_2799121 Anti-IRF7 Cell Signaling Technology Cat# 4920; RRID: AB_2127551 Anti-STAT1 Cell Signaling Technology Cat# 9172; RRID: AB_2198300 Anti-Phospho-STAT1 (Tyr701) Cell Signaling Technology Cat# 9167; RRID: AB_561284 Anti-STAT2 Cell Signaling Technology Cat# 4594; RRID: AB_2271323 IFIT1 Abcam Cat# 236256 Anti-Phospho-STAT2 (Tyr690) Cell Signaling Technology Cat# 88410; RRID: AB_2800123 Anti-Beclin 1 Cell Signaling Technology Cat# 3495; RRID: AB_1903911 Anti-ATG 5 Cell Signaling Technology Cat 12994; RRID: AB_2630393 Anti-ATG 12 Cell Signaling Technology Cat# 4180; RRID: AB_1903898 Anti-LC 3 Cell Signaling Technology Cat# 2775; RRID: AB_915950 Anti-LAMP1 Cell Signaling Technology Cat# 9091; RRID: AB_2687579 Anti-LAMP2 Abcam Cat# 25631 Anti-Capthesin B Cell Signaling Technology Cat#31718; RRID: AB_2687580 Anti-Capthesin D Abcam Cat# ab198326 Anti-Rab 11 Cell Signaling Technology Cat# 5589; RRID: AB_10693925 Anti-Rab 8b Thermo Fisher Scientific CAT# PA5-67354; RRID: AB_2664303 Anti-Flag-HRP Sigma Aldrich Cat# A8592; RRID: AB_439702 Anti-HA-HRP Abcam Cat# ab128131; RRID: AB_11143947 Anti-c-Myc Roche Cat# 11667203001; RRID: AB_390911 Bacterial and Virus Strains A/California/07/2009(H1N1 pandemic) Jahan et al.9 N/A A/Oklahoma/309/06 (H3N2) Fan et al.43 N/A A/Hong Kong/1073/99 (H9N2) Chan et al.44 N/A A/Vietnam/1203/2004 (H5N1) Herfst et al.45 N/A A/Shanghai/02/2013 (H7N9) Mok et al.46 N/A Human coronavirus 229E ATCC VR-740 BetaCoV/Hong Kong/VM20001061/2020 Lv et al.47 N/A ZIKV (NC-14-5132) Lan et al.16 and Li et al.17 N/A Chemocompetent E. coli DH5a Thermo Fisher Scientific Cat# 18265017 (Continued on next page) e1 Developmental Cell 58, 2495–2509.e1–e6, November 20, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins DMEM Thermo Fisher Scientific Cat# 10569010 HEPES Thermo Fisher Scientific Cat# 15630130 Trypsin-EDTA Thermo Fisher Scientific Cat# 25200072 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122 Fetal Bovine Serum (FBS) Thermo Fisher Scientific Cat# 10500064 Phosphate-buffered saline (PBS) Thermo Fisher Scientific Cat# 14040133 Puromycin InvivoGen Cat# ant-pr-1 Hygromycin B Thermo Fisher Scientific Cat# 10687010 Triton X-100 Sigma Aldrich Cat# 93443 Complete protease inhibitor cocktail Roche Cat# 11836145001 Normal Goat Serum (NGS) Thermo Fisher Scientific Cat# 31873 MG132 Sigma Aldrich Cat# M7449 TransITLT1 transfection reagent Mirus Cat# MIR2304 Lipofectamine 3000 Thermo Fisher Scientific Cat# L3000008 Polyethylenimine (PEI) Polysciences Cat# 23966-1 DharmaFECT1 Dharmacon Cat# T-2001-03 Octadecyl Rhodamine B Chloride (R18) Thermo Fisher Scientific Cat# O246 Fix Perm Kits BD PharMingen Cat# 554714 TPCK-Trypisn Thermo Fisher Scientific Cat# 20233 T4 ligase New England Biolabs Cat# M0202S Endo H New England Biolabs Cat# P0702S PNGase F New England Biolabs Cat# P0704S Critical Commercial Assays TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.USP28.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.SMURF1.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.BECN1.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.MAP1LC3B.13 PrimeScript RT Reagent Kit TAKARA Cat# RR037A MiniBEST Universal RNA Extraction Kit TAKARA Cat# 9767 MiniBEST Viral RNA Extraction Kit TAKARA Cat# 9766 TB Green Premix Ex Taq II (Tli RNase H Plus) TAKARA Cat# RR820A LEGENDplex Human Anti-Virus Response Panel Biolegend Cat# 740390 LEGENDplex Human Proinflammatory Chemokine Panel 1 Biolegend Cat# 740984 Deposited Data Proteomics; PRIDE database This study Accession: PXD034797 Experimental Models: Cell Lines ..

    Article Title: Usp25-Erlin1/2 activity limits cholesterol flux to restrict virus infection.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Usp25 Abcam Cat# ab187156 Anti-SMURF1 Santa Cruz Cat# sc-100616 Anti-Usp28 Abcam Cat# ab126604; RRID: AB_11127442 Anti-GAPDH Abcam Cat# ab8245; RRID: AB_2107448 Anti-PB2 Thermo Fisher Scientific Cat# PA5-32220; RRID: AB_2549693 Anti-HA Abcam Cat# ab119966; RRID: AB_10899358 Anti-NS1 Santa Cruz Cat# sc-130568; RRID: AB_2011757 Anti-NP Abcam Cat# ab128193; RRID: AB_11143769 Anti-RIG-I Abcam Cat# ab180675 Anti-MAVS Cell Signaling Technology Cat# 3993; RRID: AB_823565 Anti-TBK1 Cell Signaling Technology Cat# 3504; RRID: AB_2255663 Anti-Phospho-TBK1 (Ser172) Cell Signaling Technology Cat# 5483; RRID: AB_10693472 TRAF3 Abcam Cat# 36988 Anti-IRF3 Cell Signaling Technology Cat# 4302; RRID: AB_1904036 Anti-Phospho-IRF3 (Ser386) Cell Signaling Technology Cat# 37829; RRID: AB_2799121 Anti-IRF7 Cell Signaling Technology Cat# 4920; RRID: AB_2127551 Anti-STAT1 Cell Signaling Technology Cat# 9172; RRID: AB_2198300 Anti-Phospho-STAT1 (Tyr701) Cell Signaling Technology Cat# 9167; RRID: AB_561284 Anti-STAT2 Cell Signaling Technology Cat# 4594; RRID: AB_2271323 IFIT1 Abcam Cat# 236256 Anti-Phospho-STAT2 (Tyr690) Cell Signaling Technology Cat# 88410; RRID: AB_2800123 Anti-Beclin 1 Cell Signaling Technology Cat# 3495; RRID: AB_1903911 Anti-ATG 5 Cell Signaling Technology Cat 12994; RRID: AB_2630393 Anti-ATG 12 Cell Signaling Technology Cat# 4180; RRID: AB_1903898 Anti-LC 3 Cell Signaling Technology Cat# 2775; RRID: AB_915950 Anti-LAMP1 Cell Signaling Technology Cat# 9091; RRID: AB_2687579 Anti-LAMP2 Abcam Cat# 25631 Anti-Capthesin B Cell Signaling Technology Cat#31718; RRID: AB_2687580 Anti-Capthesin D Abcam Cat# ab198326 Anti-Rab 11 Cell Signaling Technology Cat# 5589; RRID: AB_10693925 Anti-Rab 8b Thermo Fisher Scientific CAT# PA5-67354; RRID: AB_2664303 Anti-Flag-HRP Sigma Aldrich Cat# A8592; RRID: AB_439702 Anti-HA-HRP Abcam Cat# ab128131; RRID: AB_11143947 Anti-c-Myc Roche Cat# 11667203001; RRID: AB_390911 Bacterial and Virus Strains A/California/07/2009(H1N1 pandemic) Jahan et al.9 N/A A/Oklahoma/309/06 (H3N2) Fan et al.43 N/A A/Hong Kong/1073/99 (H9N2) Chan et al.44 N/A A/Vietnam/1203/2004 (H5N1) Herfst et al.45 N/A A/Shanghai/02/2013 (H7N9) Mok et al.46 N/A Human coronavirus 229E ATCC VR-740 BetaCoV/Hong Kong/VM20001061/2020 Lv et al.47 N/A ZIKV (NC-14-5132) Lan et al.16 and Li et al.17 N/A Chemocompetent E. coli DH5a Thermo Fisher Scientific Cat# 18265017 (Continued on next page) e1 Developmental Cell 58, 2495–2509.e1–e6, November 20, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins DMEM Thermo Fisher Scientific Cat# 10569010 HEPES Thermo Fisher Scientific Cat# 15630130 Trypsin-EDTA Thermo Fisher Scientific Cat# 25200072 Penicillin-Streptomycin Thermo Fisher Scientific Cat# 15140122 Fetal Bovine Serum (FBS) Thermo Fisher Scientific Cat# 10500064 Phosphate-buffered saline (PBS) Thermo Fisher Scientific Cat# 14040133 Puromycin InvivoGen Cat# ant-pr-1 Hygromycin B Thermo Fisher Scientific Cat# 10687010 Triton X-100 Sigma Aldrich Cat# 93443 Complete protease inhibitor cocktail Roche Cat# 11836145001 Normal Goat Serum (NGS) Thermo Fisher Scientific Cat# 31873 MG132 Sigma Aldrich Cat# M7449 TransITLT1 transfection reagent Mirus Cat# MIR2304 Lipofectamine 3000 Thermo Fisher Scientific Cat# L3000008 Polyethylenimine (PEI) Polysciences Cat# 23966-1 DharmaFECT1 Dharmacon Cat# T-2001-03 Octadecyl Rhodamine B Chloride (R18) Thermo Fisher Scientific Cat# O246 Fix Perm Kits BD PharMingen Cat# 554714 TPCK-Trypisn Thermo Fisher Scientific Cat# 20233 T4 ligase New England Biolabs Cat# M0202S Endo H New England Biolabs Cat# P0702S PNGase F New England Biolabs Cat# P0704S Critical Commercial Assays TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.USP28.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.SMURF1.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.BECN1.13 TriFECTa DsiRNA Kit Integrated DNA Technologies Cat# hs.Ri.MAP1LC3B.13 PrimeScript RT Reagent Kit TAKARA Cat# RR037A MiniBEST Universal RNA Extraction Kit TAKARA Cat# 9767 MiniBEST Viral RNA Extraction Kit TAKARA Cat# 9766 TB Green Premix Ex Taq II (Tli RNase H Plus) TAKARA Cat# RR820A LEGENDplex Human Anti-Virus Response Panel Biolegend Cat# 740390 LEGENDplex Human Proinflammatory Chemokine Panel 1 Biolegend Cat# 740984 Deposited Data Proteomics; PRIDE database This study Accession: PXD034797 Experimental Models: Cell Lines ..



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